Turbo End Repair (Blunting) and Quick Ligation Kit

The Turbo End Repair (Blunting) and Quick Ligation Kit is optimized to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.

Components

• DNA Blunting Enzyme Mix
• DNA Ligation Enzyme Mix
• 10x Blunting Buffer
• 2 mM dNTP Solution
• Nuclease Free Water

Storage and Stability

The kit components should be stored at -20°C.  If stored at recommended temperature, the kit components are stable for six months.

DISCLAIMER

For Research Use Only. Not for Use in Diagnostic Procedures.