Pre PCR Repair Mix

The Pre-PCR Repair Mix contain cocktail of enzymes to repair damaged DNA to be used in polymerase chain reaction (PCR), micro-arrays and other applications.  The enzyme cocktail will repairs broad range of damaged DNA such as:

• Nicks - Caused by Hydrolysis and Nuclease Shearing
• Blocked 3'-Ends - Multiple Causes
• Oxidized Pyrimidines - Caused by Oxidation
• Damaged Cytosine - Caused by Hydrolysis
• Oxidized Guanine - Caused by Oxidation
• Abasic Sites - Caused by hydrolysis
  
  

In addition Pre-PCR Mix will also remove a variety of moieties from the 3'-end leaving OH group.  The enzyme cocktail will NOT repair 8-oxo-7,8-dihydro-2'-deoxyadenosines or DNA that are fragmented and has protein DNA crosslinks.

Applications and Highlights

• To repair damaged DNA prior to PCR
• Easy to use protocol

Kit Components

• Pre-PCR Repair Mix
• 10x Reaction Buffer
• 100x NAD+ Solution
• Control Damaged Lambda DNA
• Control Lambda PCR Primer
• Nuclease Free Water
• Instruction Manual

Storage and Stability

Upon arrival, the kit components should be stored at -20°.  The kit components are stable for SIX months if stored at recommended temperature,

DISCLAIMER

For Research Use Only. Not for Use in Diagnostic Procedures.