Human Normal Tissue Lysate
Tissue Lysates are Ideal for biomarker and antibody screening/ characterization, protein-protein interaction, protein expression, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting. We provide tissue lysates from the following tissues:
Brain • Breast • Colon • Heart • Kidney • Liver • Lung • Ovary • Pancreas • Prostate • Placenta • Spleen • Stomach • Testis
All human materials are obtained under strict bioethical standards using IRB-approved protocols that ensure patient confidentiality, safety and informed consent.
Normal Human Tissues were collected and flash frozen within 5-10 minutes of removal. Tissue total protein lysates are prepared from whole tissue homogenates by homogenization in modified RIP buffer along with protease and phosphatase inhibitor cocktail, and provided at a concentration of 2-5 mg/ml.
The quality of protein is tested by SDS-PAGE with coomassie blue staining of the gel. Proteins from one of gel was transferred to PVDF membrane and tested with beta actin antibody.
• Tissue Lysates (2-5 mg ⁄ ml)
• Instruction Manual
Storage and Stability
From time of receipt, this product is stable for SIX months at – 20°C or 12 months at – 70°C.
FOR IN - VITRO RESEARCH USE ONLY - NOT RECOMMENDED FOR DIAGNOSTIC OR THERAPEUTIC USAGE