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qRT-PCR Master Mix


Reverse Transcription and qPCR in One Reaction

The qRT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining Reverse Transcriptase and Hot Start Taq Probe qPCR Master Mix in a single-step real-time amplification reaction.

The qRT-PCR Mix for 1-Step RT-qPCR (50X) combines optimized quantities of Reverse Transcriptase, RNase Inhibitor and additives to enhance single-step reactions. The Hot Start Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination.  The kit has the following advantages:

  • Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
  • Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
  • Resistant to most PCR inhibitors



For In-Vitro Research Use Only. Not for Diagnostic or Therapeutic Use.

qRT-PCR Master Mix