qRT-PCR Master Mix
Reverse Transcription and qPCR in One Reaction
The qRT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining Reverse Transcriptase and Hot Start Taq Probe qPCR Master Mix in a single-step real-time amplification reaction.
The qRT-PCR Mix for 1-Step RT-qPCR (50X) combines optimized quantities of Reverse Transcriptase, RNase Inhibitor and additives to enhance single-step reactions. The Hot Start Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing users to incorporate UNG into subsequent reactions for control of possible carryover contamination. The kit has the following advantages:
- Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
- Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
- Resistant to most PCR inhibitors
For In-Vitro Research Use Only. Not for Diagnostic or Therapeutic Use.