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Turbo End Repair (Blunting) and Quick Ligation Kit
The Turbo End Repair (Blunting) and Quick Ligation Kit is optimized to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.
Components
- DNA Blunting Enzyme Mix
- DNA Ligation Enzyme Mix
- 10x Blunting Buffer
- 2 mM dNTP Solution
- Nuclease Free Water
Storage and Stability
The kit components should be stored at -20°C. If stored at recommended temperature, the kit components are stable for six months.
DISCLAIMER
For Research Use Only. Not for Use in Diagnostic Procedures.
Turbo End Repair (Blunting) and Quick Ligation Kit PRODUCTS
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