Ultra Taq DNA Polymerase

Ultra Taq DNA polymerase is isolated from the E. coli Thermus aquaticus. The molecular weight of the product is approximately 94 kDa. Ultra Taq DNA polymerase has the 5′- to 3′- DNA polymerase activity and 5′- to 3′- exonuclease activities without 3′-5′- exonuclease activity. The extending speed is 1-2 kb/ min. This adds extra "A" on 3′- end, therefore, the PCR product can be cloned in TA vector.

Components

• Ultra Taq DNA Polymerase - 5 U/μl
• 10x Ultra Taq Buffer
• PCR Grade Water
• Instruction Manual

Highlights

• High-sensitivity
• High amplification efficiency
• Lower error rate than regular Taq Polymerase

Unit Definition: One unit of Ultra Taq DNA Polymerase High Fidelity incorporates 10 nmoles of deoxyribonucleotides into acid-perceptible material in 30 minutes at 74°C. Quality Control: This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of Ultra Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA

Storage Buffer: 20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA,1 mM DTT,100 mM KCl, Stabilizers and 50% glycerol.

10X PCR reaction Buffer: 500 mM KCl,100 mM Tris-Cl (pH8.5 at 25℃),1% Triton X-100,15 mM MgCl2

 Storage and Stability

From time of receipt, this product is stable for SIX months if stored at at – 20°C.

DISCLAIMER

For Research Use Only. Not for Use in Diagnostic Procedures.