Tissue Lysates & Blots
Luminous BioSciences provides ready-to-use Protein Lysates isolated from human (normal and tumor) and mouse tissues.
Human tissue samples were obtained from U.S. medical centers using the industry's most strict ethical requirements and IRB protocols. The diagnosis of every tissue sample, whether cancer, normal or other disease, has been verified by an independent board-certified pathologist.
Tissue protein lysates are prepared by grinding tissues in modified RIPA Buffer in the presence of protease and phosphatase inhibitors. After lysis, the lysates are sonicated and centrifuged at high speed to remove cell debris. Protein concentration was determined by BCA Protein Assay. For quality control purposes, the lysate was electrophoresed on 4-20% PAGE and stained with coomassie blue.