- Home
- LBS Services
- Plasmid DNA Manufacturing Services
Plasmid DNA Manufacturing Services
Transfection Grade Plasmid DNA production - for Research use
Pharmaceutical Pre-clinical Grade Plasmid DNA production
Luminous Biosciences offers customized transfection grade and pharma grade plasmid midi prep (1 mg), maxi prep (5 mg), giga prep (10 mg), small and large-scale (100 mg – 10 gram quantity) plasmid DNA isolation services that will be endotoxin free and thus will be suitable for transfection and animal studies to both biotechnology researchers and pharmaceutical companies. Bacterial cultures containing plasmids are grown in special media and Plasmid DNA is isolated using Luminous Bio’s proprietary technology that yields high quality plasmid DNA.
LUMINOUS BIOSCIENCES Plasmid Production Project Outline:
OUR advantages
- High transfection efficiency plasmid DNA
- Isolated Plasmid DNA is guaranteed to have very low endotoxin
- Guaranteed Yield for High Copy plasmid DNA (Low Copy plamids have extra charge based on yield)
- Fast turn around time (4+ days depending on the required yield and the type of plasmid preparation)
- Custom Plasmid DNA will be Free of RNA, Chromosomal DNA and Proteins
- Plasmid DNA is supplied in choice of your formulation and concentration
Typical quality control specifications for pharma grade plasmid DNA
- Identity of the plasmid DNA by Restriction Digestion and DNA Sequencing*
- Purity of the Plasmid DNA by A260/A280 Ratio
- Residual E. coli (host) RNA and DNA Comtamination Test by PCR*
- Residual Protein levels by Bradford Assay
- Endotoxin test by LAL Assay
- Sterility (Bioburden)* for Microbial Contamination
* Bioburden Assay and E. coli DNA contamination Test by PCR are performed for pharma grade plasmid DNA - additional charges apply
Sample Submission Requirements
- Plasmid DNA (2-5 μg) or Glycerol Stock
- Restriction map and Antibiotic resistance information
Luminous Plasmid Sample Data Sheet
Luminous Plasmid Order Form
Additional Services
- Production & Analysis of Master Cell Bank
- Third Party Confirmation of Results
- Custom Formulations and Packaging
Please e-mail or call us about your custom plasmid prep inquiries as follows:
Contact Us
North America: Tel: +1-301-296-8436 Toll Free +1-800-997-2610
Europe: Tel +44-121-227-5283 Toll Free: 0800-015-4624