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Expression & Protein Purification Services
Outsource Your Expression and Recombinant Protein Purification Needs
Luminous BioSciences offers custom cloning, expression and recombinant protein purification services in E. coli.
Phase I: Sub cloning
- Sub cloning of your cDNA into bacterial expression vector with His-tag or GST-tag
Phase II: Assessment and Optimization of Expression
- Expression optimization by transformation into two E. coli host cells and expression optimization at two different temperatures
- Time course study of expression
- Small scale protein purification and QC by SDS-PAGE and western blot analysis
Phase III: Recombinant Protein Purification
- Large Scale growth of best E. coli expressing construct
- Harvesting cells and cell lysis
- Protein purification by affinity tag resins
- Secondary purification on anion exchange resin or by gel filtration (additional charges applies)
- Dialysis and final QC by SDS-PAGE (>85-90% purified protein)
To discuss custom expression and protein purification project please send inquiries or contact us as follows:
Contact Us
North America: Tel: +1-301-296-8436 Toll Free +1-800-997-2610
Europe: Tel +44-121-227-5283 Toll Free: 0800-015-4624