Toll Free: 800 - 997 - 2610
Local: 301 - 296 - 8436

Expression & Protein Purification Services

Outsource Your Expression and Recombinant Protein Purification Needs

Luminous BioSciences offers custom cloning, expression and recombinant protein purification services in E. coli.

Phase I:
Sub cloning

  • Sub cloning of your cDNA into bacterial expression vector with His-tag or GST-tag

Phase II: Assessment and Optimization of Expression

  • Expression optimization by transformation into two E. coli host cells and expression optimization at two different temperatures
  • Time course study of expression
  • Small scale protein purification and QC by SDS-PAGE and western blot analysis

Phase III: Recombinant Protein Purification

  • Large Scale growth of best E. coli expressing construct
  • Harvesting cells and cell lysis
  • Protein purification by affinity tag resins
  • Secondary purification on anion exchange resin or by gel filtration (additional charges applies)
  • Dialysis and final QC by SDS-PAGE (>85-90% purified protein)

    To discuss custom expression and protein purification project please send inquiries or contact us as follows:

Contact Us

North America: Tel: +1-301-296-8436 Toll Free +1-800-997-2610

Europe: Tel +44-121-227-5283 Toll Free: 0800-015-4624

Expression & Protein Purification Services WE OFFER

Name Catalog Price Quantity    
Cloning and Expression Optimization
LS9160 $0.00 Quote Request
Protein Purification
LS9161 $0.00 Quote Request