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NGSUltra™ Pre-PCR Repair Mix

The NGSUltra™ Pre-PCR Repair Mix contain cocktail of enzymes to repair damaged DNA to be used in polymerase chain reaction (PCR), micro-arrays and other applications.  The enzyme cocktail will repairs broad range of damaged DNA such as:

  • Nicks - Caused by Hydrolysis and Nuclease Shearing
  • Blocked 3'-Ends - Multiple Causes
  • Oxidized Pyrimidines - Caused by Oxidation
  • Damaged Cytosine - Caused by Hydrolysis
  • Oxidized Guanine - Caused by Oxidation
  • Abasic Sites - Caused by hydrolysis

In addition NGSUltra™ Pre-PCR Mix will also remove a variety of moieties from the 3'-end leaving OH group.  The enzyme cocktail will NOT repair 8-oxo-7,8-dihydro-2'-deoxyadenosines or DNA that are fragmented and has protein DNA crosslinks.

Applications and Highlights

  • The kit components has been tested to perform in all NextGen Sequencing applications
  • To repair damaged DNA prior to PCR or other applications
  • Easy to use protocol

Kit Components

  • Pre-PCR Repair Mix
  • 10x Reaction Buffer
  • 100x NAD+ Solution
  • Control Damaged Lambda DNA
  • Control Lambda PCR Primer
  • Nuclease Free Water
  • Instruction Manual

Storage and Stability

Upon arrival, the kit components should be stored at -20°.  The kit components are stable for SIX months if stored at recommended temperature,

 

 

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.

NGSUltra™ Pre-PCR Repair Mix
NGSUltra™ Pre-PCR Repair Mix PRODUCTS
Product Catalog Size Price Qty  
NGSUltra Pre PCR Repair Mix
NGD010-1 40 Rxns $144.00
(WAS $160.00)
NGSUltra Pre PCR Repair Mix
NGD010-2 150 Rxns $472.50
(WAS $525.00)